LOCUS BG067621 686 bp mRNA linear EST 26-JAN-2001 DEFINITION H3056D06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056D06 3', mRNA sequence. ACCESSION BG067621 VERSION BG067621.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 686) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3056D06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: D column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..686 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056D06-3" /db_xref="taxon:10090" /clone="H3056D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 195 c 185 g 159 t ORIGIN 1 ccgcccgctc ccaagatcca actacgagct ttttaactgc agcaacttta atatacgcta 61 ttggagctgg aattaccgcg gctgctggca ccagacttgc cctccaatgg atcctcgtta 121 aaggatttaa agtggactca ttccaattac agggcctcga aagagtcctg tattgttatt 181 tttcgtcact acctccccgg gtcgggagtg ggtaatttgc gcgcctgctg ccttccttgg 241 atgtggtagc cgtttctcag gctccctctc cggaatcgaa ccctgattcc ccgtcacccg 301 tggtcaccat ggtaggcacg gcgactacca tcgaaagttg atagggcaga cgttcgaatg 361 ggtcgtcgcc gccacggggg gcgtgcgatc ggcccgaggt tatctagagt caccaaagcc 421 gccggcgccc gacccccggc cggagccggg agggagctca ccgggttggt tttgatctga 481 taaatgcacg catccccccc cgggaagggg ggtcagcgcc cgtcggcatg tattagctct 541 agaattacca cagttatcca agtaggagag gagcgagcga ccaaaggaac cataactgat 601 ttaatgagcc attcgcagtt tcactgtacc ggccgtgcgt acttagacat gcatggctta 661 atcttttcga ctatccggat agtcga //