LOCUS BG067558 546 bp mRNA linear EST 12-MAY-2010 DEFINITION H3055G01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3055G01 3', mRNA sequence. ACCESSION BG067558 VERSION BG067558.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 546) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067558.1. Other_ESTs: H3055G01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3055 row: G column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..546 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3055G01-3" /db_xref="taxon:10090" /clone="H3055G01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 137 a 111 c 113 g 185 t ORIGIN 1 atacagacag gtatagatgc caagtatgtg tcacctcaca cctcagtaat ttatttcatt 61 tttgagaggg tttcactatg tggctctggc tgacctggaa cttgtcatgc agattaggct 121 ggcctggagc tcacaagtgc tgggatcaca ggtgtgcacc atcattcctg gcttgcatgt 181 taaaacagca ttggtgttgc tgaatgtgaa tgaagaagta tctgtgagga tctgggtctg 241 ctgtggctcc tgccggcctg agggcccttt tggtgatcag catgcccttg gatgagatca 301 tattttcaaa ggtattctct aggttgtaat gtctgaatcc ttttcaagtt ggctttgaac 361 ttttcctgaa tcactatgct gagacttatc tagatttttc cttctatatg ctttcccaca 421 taaaaaaaaa aacttgttat tgattatatt ttgactcttt tgaacttttt cctaaaatga 481 atagaataca ctgtaactaa gtccatttct cctcccaccc catttttctt ccagataagg 541 aggaaa //