LOCUS BG067457 673 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054F06 3', mRNA sequence. ACCESSION BG067457 VERSION BG067457.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 673) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067457.1. Other_ESTs: H3054F06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: F column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..673 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054F06-3" /db_xref="taxon:10090" /clone="H3054F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 226 a 150 c 135 g 162 t ORIGIN 1 acttaaagtt cataaaaaaa taggttctgt atttagcttc aaaaaaaaaa aaaaacctat 61 cccagtgcaa cttaaatgaa aaaagacttc tttaaaaaaa aaaaaaacag ccagtagcag 121 caaaacaatg tcaaacattg taaacaaacc cttatcaaca accctatacc taataacaaa 181 acttccatac tccttgaaag cattaaatgc gtacaagagg gcaattttgg tcctcttttt 241 cttactacaa aaatacttag tatctgaaac aatgaatgtt accaggagca gacacaatgt 301 ggtaacatca tgagaggttt ggaacaactt tgggccatca tgttgctgca gtgggcaagc 361 tctccaaatg ttgtctccgg gctatgatcc tggcacagcg cgggcatctc gaagactgga 421 aacactgttt gtgaaagcag gccctgcacg ctgcacatct tctacaagtc gtggtctgaa 481 atgggaagat gaccgtcgtg ctctgacaaa actcacaaat gaagcccttc ccctggcaca 541 gctcacagct gtccacgtgg gcaagagaag ctctcaggat gtctttaagc aaaggtgcca 601 acagtccttt cttggtcctg aggaagtcgt ccatggagaa tatgtggcat tcatcagtca 661 agtggctggg aac //