LOCUS BG067415 822 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054B10 3', mRNA sequence. ACCESSION BG067415 VERSION BG067415.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 822) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067415.1. Other_ESTs: H3054B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..822 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054B10-3" /db_xref="taxon:10090" /clone="H3054B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 209 a 172 c 186 g 255 t ORIGIN 1 ggaaactttt tttatttgat atttaagtaa gatcaaaaac tacaagattt tttttttttg 61 ttttgttttt gaggctgatg agtgcaaaga gaaacgctaa acatcagtta tcacctgatt 121 agctcgctga ggtgctcggg gctcagacta ctcagtgaca taccagttac tgtgtgtggc 181 ttgagaatct tgcagcaaaa agtatcctaa atgaaacact gggctagggg ctgtggagtc 241 tgagtaggag cggtggtggc agggtggcct tgggattctt ctaggctcaa cagctggtta 301 ccctacacaa ggacctagca tccagctatt tcatgtttga ctttttcagg ttaaacaact 361 taactcaaaa cctcttagca gaaataaaga ctatacacgg tctgaaaaca taaagctgac 421 ccacttctcc agcagcagtc tcgctgagtg gagtcctaag gaaacagtga gaatctgcac 481 tgctcgctac cctacatctg ctgcgtggag tttccattcc aagtgttgtt ctcgtcttca 541 ccgtcatctt gagtcctcag tctgtatatt ttcccttctt ttttaaagtc ttcccctcgt 601 ttttccagca ttctttttcg gactggttcc cttttctctg atttgctgga tgatgcagag 661 tttgaaggct ctgtggatgt ggctcttgct gaagtctgtg caggaggagg attgctaaat 721 aaaaaattgc taattaatct ccatatggcc aggaatggat aaagcaccgt ccccagtaat 781 gtccaaatgt ctccactaga agagtggaca atggatgtgg ct //