LOCUS BG067329 809 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053B10 3', mRNA sequence. ACCESSION BG067329 VERSION BG067329.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 809) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067329.1. Other_ESTs: H3053B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..809 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053B10-3" /db_xref="taxon:10090" /clone="H3053B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 239 a 180 c 176 g 214 t ORIGIN 1 ctctactctc caccataaaa tatcactgag cctaggctct atcgccgtcg tctttcactg 61 gctttggtca aaggaatagt ctagatttct ggacaaaaaa aattctatct aatgtttcct 121 accctgcttc tttattcaga tttatattta gaaaaactgt tgatgaactg tttgagaatc 181 acagaagcat gtagaggtat ggggaaatta tagaacacag cgcatgcatc atactgaaaa 241 ctttggcttc tcataagtct ctgattttaa accaaggctt gggaaatgag gaaggagagt 301 gtgagattaa ggtatctaga aaaggcaact cctatgccag tatgtgatga tgctctgtgc 361 ctagaatccc aatactgtag cagctgacgc acagaggatt atgtgttcaa ggccatcctg 421 ggctggatag taagactctg tgcacccccc caaaaaaatc aaatgttaag tcaagatcaa 481 cctgatctca aagggatggt tgtttggcta gtgtgactaa tctgcacttg cttgtgggac 541 cctagcacac actcagagga tatgtaatga aacatgttgg ggaaagtcac atagaagcct 601 ggcctagggt agatgacaag gctttgaagg ggtgcccccc cccactgcaa tgagcattca 661 tgggagcata aggagttaga aaacttccca cactccctcc taaatccccg atactgttcg 721 aacagagcaa cctgacatca ttatggcctc gtaactctca aattcaaggc catcctgggc 781 ataactggtc cattcactca gtcagtctg //