LOCUS BG067131 693 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050H10 3', mRNA sequence. ACCESSION BG067131 VERSION BG067131.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 693) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067131.1. Other_ESTs: H3050H10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: H column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..693 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050H10-3" /db_xref="taxon:10090" /clone="H3050H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 178 c 193 g 178 t ORIGIN 1 aaatttttgg gtaaaataaa accttttatt ctcaactcca atgactgctg cctcccaggt 61 accccatctt atatcagtca ttggtgttgg gggcggggga cagaatcagc cctcctgacc 121 acaccaggct ttgtggagct aggctatgct ttgtagggtc ttgttaccag cgaaccccaa 181 gctttctgta gacacacttg ctgattgacg tatttcctgt aagttggtag cgggagggtc 241 agctagatcc gctggccagc actgagccca cgctctgccc aggccgccct taccaggatc 301 acaatgtttt ctgagatggt tcaggatgga ggccaccaac catcctgtgt tatgatgggc 361 agaggaccct cctgctttct attctcagcc acatttccag catccttggc tccataagtc 421 catgaagggt tggcttgggg tgtggagcaa ggggaggacc ctcagctgtc tgggtgtcac 481 cacctgctct ggattcttag gcctgtgttt gcctgccaga cctggtctag gtggcagatg 541 gtcacataaa aatagctcca aagtccttaa tggaccactt ccccggctgt tcttctgagt 601 ggaaggcagc tgagagggtg gaggaggatc aaccccagct aaggcccgag ggtggtgcca 661 gctgctctga gtatcattgc tgtggggcgg tga //