LOCUS BG067091 645 bp mRNA linear EST 12-MAY-2010
DEFINITION H3050E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3050E02 3', mRNA sequence.
ACCESSION BG067091
VERSION BG067091.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 645)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067091.1.
Other_ESTs: H3050E02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3050 row: E column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..645
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3050E02-3"
/db_xref="taxon:10090"
/clone="H3050E02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 173 a 171 c 125 g 176 t
ORIGIN
1 acagtttatt gtttcttcca tagcattcag gtgtgcaaga gagttcacaa gacaagccag
61 ccagctcttc acaaaccaac gggctcttga acccacagag agacaaaagc gtgagagtag
121 agatgttagc gcggagtccc gcctcagcac agtcctcagg ccctgctgca cacctccaca
181 cggcacgaaa tgcctgtgtg agctgagctc agctcctcaa accaagccct gtttcatgca
241 cagactattt catgcctggc ttccttaata aaactaccat ttcccccacc cccccccagc
301 aggaaaaaaa gtttttgttt ttaacaaacc taagctacag gaaaatgcaa gttggtaagc
361 tttgcttttg cataatataa agacagtcat ggggagttca tctcaacatc aaaccaagta
421 gatagcatcc acagctctta acttcctaac ttaatcacaa atcagtactt aggatcgaaa
481 ggtaactgcc ccagttctag ctcaagattt gccgtgtgtc ttccgttagc gcgaccgtat
541 ttttgccatt taccttccct ttcataaggc tgtggatgaa ggtgcttctg ctcttgtttt
601 cggtgcctgg cgttttcaat agttaccata cgttgctttt tatca
//