LOCUS BG067068 636 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050B12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050B12 3', mRNA sequence. ACCESSION BG067068 VERSION BG067068.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 636) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067068.1. Other_ESTs: H3050B12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: B column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..636 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050B12-3" /db_xref="taxon:10090" /clone="H3050B12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 169 c 154 g 136 t ORIGIN 1 gaaatggggt ttctctatgt agttctggct gtcctggaac tcagagagat ccacctgcct 61 ttgcctcccg agtgctaggg ttagaggata taccaccacg cctggacctc aaactcgaac 121 tcataatctt atatgaactc ttagaataaa agaaaagata gaatatgtct caaattgcat 181 caataccagc ataaccttga taccagtgct aaggctgtgg ctcggttggg agagcggtga 241 cctacacggc ctgggttgat ccccagcagg gcaaagccaa gtgcacttct agcccagggg 301 aggaagaagc gggagaacca caagtccaac tgacagatca gttcactgac aggttagtcc 361 actgacaggt cagtccactg acaggtcagt ccactgacag gttagtccac tgacagatca 421 gtccactgac aggtcagtcc actgacaggt tagtccactg acagatcagt ccactgacag 481 attagtccac tgacaggtca ttccactgac aggtcagtcc actgacaggt cagtccacag 541 acaggtcagt ccactgacag atcagtccac tgacaggtca gtccactgac aggtcagtcc 601 tctgacaggt cagtccactg agagatcagt ccactg //