LOCUS BG067049 728 bp mRNA linear EST 12-MAY-2010
DEFINITION H3050A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3050A04 3', mRNA sequence.
ACCESSION BG067049
VERSION BG067049.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 728)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067049.1.
Other_ESTs: H3050A04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3050 row: A column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..728
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3050A04-3"
/db_xref="taxon:10090"
/clone="H3050A04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 243 a 164 c 141 g 180 t
ORIGIN
1 ctcgataaag ccatggatga ttgtaaatgc tacctccaaa ctcatcaccc gggctgaggc
61 tttgtccaca gaggagatct ttccagctcc atcggctagc acttggtctt gagcagattt
121 cttcagattc aactcagttc aacaacaaca acaacaaaaa gcaaccaaaa aacctgagct
181 ggttagctac aaatggagaa aataaagcct ccacacaaaa aaggaatgtt aagacaaaat
241 tctattcaag aaatccacca atacagcatt aatcctatca cgtttcacag cattaactat
301 gcatccaatg aaatccgtct gcaggaaatc ctaacggctc ccactgatca ggcagagcgg
361 ctgtctaaag tctgcacttg tccacacctg ttttgctgaa cacaggagag atatcctgtt
421 tgtaactcag atgtccattt acccagtcaa gtaaatttca gtaaatcttg ctcttacagg
481 actattaaga ccagaaactt tcccttttgg agcctttagt tcaactccag tttgtcaatg
541 ttaggaatag tctagcatct gatacagagc taggtttgac aagacactga agaatttaag
601 taaaggcgtg gtgaaatgga gcagggagaa agggagattt gtatcaagag ttaaaactga
661 agccaaagtg tcgtcttact gcataataca gtaacaactg caaacgcttt acaagcaagg
721 gggcccac
//