LOCUS BG067017 821 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049F05 3', mRNA sequence. ACCESSION BG067017 VERSION BG067017.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 821) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067017.1. Other_ESTs: H3049F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..821 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049F05-3" /db_xref="taxon:10090" /clone="H3049F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 215 a 214 c 200 g 192 t ORIGIN 1 cttcaaaata ttttattata aaacacaaca cagggctcac caacagttcc cacccaacac 61 tgtcaaatac acactgctgg acacagtgcc cccaagatct gactccagtt gagaatctca 121 gtttcaggaa aaccgcagca gggagctgaa tggagagacg gcaagtcgcc aggatgctgt 181 gctgatgctc aacaagaaca gtccctccag gagtggacac ccatggccag ggctgtgggc 241 caccgagggc ttgagcccaa gtttgggcag agaggagatg aaactccaac agtcccagct 301 tcaggccact atttcttatt ccttggcttt agctcttcag ctgcattacg caagaaaatg 361 taatttttct tttggggatt ataaaactca tgtcccttgg accagtcgta gctggaagca 421 taggcaaata tgtttccatt gtgattgaag cagcaagctg cgatgggctg atctaactgt 481 tccgaagtct ttagtttggt cctggcatct ttgtcccaga agctgaacct accatcggag 541 cccacagtgg caagggtacc atggacagga tggaaggcaa tcccattcac ctggtgcttc 601 aggggagact cgattctcct gaactcagag ggctggttct ccagctgata gacaatcagg 661 cccctctctg cagtggctac cacagccatc gggtatatca catctgcaca gtaacagcgt 721 tcagggagtt gcaagaccat cataggattt gaggaccgcg tatcccagaa cttcagagtc 781 ttatcccagc tcccggtcat cacacagctg tagtttgggg c //