LOCUS BG066995 792 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049D06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049D06 3', mRNA sequence. ACCESSION BG066995 VERSION BG066995.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 792) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066995.1. Other_ESTs: H3049D06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: D column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..792 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049D06-3" /db_xref="taxon:10090" /clone="H3049D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 171 a 211 c 151 g 259 t ORIGIN 1 atgtgctcta atgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcgcacatgt gtgtgcctgg 61 tgcttgcaac aacagaagag ggaatcagag tccccagatc tgccattaca gctgcttgcc 121 agttaccatg gtctgcaaga gcagcaagtg ctcttaggct gtctctccac ccccttcccc 181 atctttcatt tgagtaataa gctgcccaaa aaagtatatg cggtgcatgt ctgtgctgca 241 tgtacgtaaa cagatgtggt tgcccatgca taatgtgtag aagccagagg tcagtgtcaa 301 ttatctttct ccattgcttt ccatcccact ctttggagac aggctctctc agtgaaactg 361 gagctcactg attggctgtg ttgactggcc aaccaacaat ggaactcact gattgcctgg 421 gctgactggc caaccagcaa gtgctgttga cttgagtcat cccccatcct ttttcttttc 481 tttttttttt tcctgccccc tccttttaaa tatactttcc ttgttcttct ttctttaatt 541 ttcttacttt tccctcttct tttctcttta cttcttttct tcctcttgta tttttccttc 601 ttctctctcc atccaccaac acccatccat ccgttaatac ttaccaagta ccttgcatat 661 tcccctcctg cacatactga gattaaggat atcaggataa ccaccaagaa taagcagttc 721 atggggggaa gtatgttcca caggaggaac acctgtccct aagtacagtc acacaagcca 781 cttgctcagt ga //