LOCUS BG066992 623 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049D03 3', mRNA sequence. ACCESSION BG066992 VERSION BG066992.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 623) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066992.1. Other_ESTs: H3049D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..623 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049D03-3" /db_xref="taxon:10090" /clone="H3049D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 152 c 139 g 176 t ORIGIN 1 cttgtagcac agatttccaa tcagacggat gagatgagac ttaaagcctt cagtcatgtg 61 ttcgatgtca ccctctgctt ttagagagtc tgagggactg aagatgttcg tgctttcttt 121 tccaacttca tgaatcactc gtaaaacatc aatcacgcgt tccatcaggc cagggaaaac 181 ctgcaggtag ccgagaagct cagtgttgga cgtcatttca cacaagacat ccaggagccg 241 aatcgtaacc agggcttcct tatcctcggt gtgaggctct gaggtcaact tcagcacgtt 301 cctgcactga tccataaatg agtttgcaat caactcggca tggcgcacaa agatagagat 361 gtcatccttt gtcagctgct cctcacccac tagcttggct atcactatgt ctaacagtgt 421 gatcctttcc tggttgctga gtttgccata catagcttcc accagttccg ggcttttcag 481 aaagtggtct gaaataatca agaacggcca ctctgacgcc gggtgctttt ggtgagcttc 541 tatgacgtta attgcaatat tgaggttctc ttccaggtct ttcatccttt cagcattaag 601 agatgtgaac agaatcatgg aac //