LOCUS BG066932 529 bp mRNA linear EST 12-MAY-2010 DEFINITION H3048F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3048F09 3', mRNA sequence. ACCESSION BG066932 VERSION BG066932.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 529) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066932.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3048 row: F column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..529 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3048F09-3" /db_xref="taxon:10090" /clone="H3048F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 115 c 157 g 119 t ORIGIN 1 cggtatcggc acatatacaa tcgtagtaga ttggcagaag agaaacaaca gattcctggc 61 tagaggggag agataaggca acgtgcatgg gggagtccaa agggggtgca gcccctctgc 121 tcctcccaca agagcttccc ctttggctgt cgcagaggca cagctgggag tgctgtgact 181 tccagctcct ggcgtctctg ctggggtgag gagtgctgtg agaagggaga caagtagttt 241 ctgcaccagt ccagctctcg gccaccaggg aaagacagag atcattccct cttttttaac 301 ttttcttttc ttggcaccat tgctttgtgg atataaggca atatgaatag taggctcaag 361 aagaagacat gtccaaggaa atagatggat ctgtacacct taagccattt gtcccatgtg 421 aagaggcaga aggcagtcat agagtaaccc atgaacagcc agtggatggt ctgttgcacc 481 aagtagtaga agggctgtag ggcagtgatg gaggccaggc tgctcaggg //