LOCUS BG066909 542 bp mRNA linear EST 12-MAY-2010 DEFINITION H3048D09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3048D09 3', mRNA sequence. ACCESSION BG066909 VERSION BG066909.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 542) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066909.1. Other_ESTs: H3048D09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3048 row: D column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..542 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3048D09-3" /db_xref="taxon:10090" /clone="H3048D09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 120 a 153 c 99 g 170 t ORIGIN 1 gtaactgttc atgtctatga tagttgtatt tggaagtaag agtaacattt ttctaaatca 61 gtgagcagtt ttatccaaag aacacatttc agaaagaata aagtgctata caaaggcctc 121 ctttccccac tcacgtaaat gatttccctt tattgggaac aatctcagtc gtgatccgca 181 gaggtcggtc ctgggcaatt cgaagataag tcttctttat cttcataggc tgtcccacac 241 caaatgcaat acaggtgctc ttctcgcaag tagccagtga ggatctgcag cttttctagc 301 acactcaaat cttcactcgg gcactcatct tcatcctgtt cttccttttc ctcttcctct 361 tcctcggtct cctcttcagg cctcagccag taccacgcct ccctgggaac ctgaatattc 421 ttctgggcat ccagctgctg gcaagctcgc tggcttcttc tcaggtctcc ttcgagcctc 481 atttcatcct gcttactctt cagtcgcatc cgaaactcct cggcggcctt tgcttcatta 541 tg //