LOCUS BG066841 676 bp mRNA linear EST 12-MAY-2010
DEFINITION H3047E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3047E12 3', mRNA sequence.
ACCESSION BG066841
VERSION BG066841.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 676)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066841.1.
Other_ESTs: H3047E12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3047 row: E column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..676
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3047E12-3"
/db_xref="taxon:10090"
/clone="H3047E12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 164 a 150 c 185 g 177 t
ORIGIN
1 ttttgttgtt tcgttttggg ggcagggctg tccatgtgga cctgaagctg attggaatag
61 agagagaatt ttgaggggtc cctgggatta aatcagtgat tctcaacatg ttcgccataa
121 cctctttggg agtcaaatgc cccttttgca ggggtcacct aagaccctca gaaaacacag
181 atatttacat tacaactcat aacagtagca caattacagt tatgaagtag ccacaaaaat
241 aattttgtgg tggtgggagg gggggtcacc acaacatgag gaactgggtt aaagggtcac
301 agcattagga gggttgagaa ccactgactt agatggagaa gaaacactgt ctcctgtgct
361 ttgggtttct ctaaagaggg aagtgtctgc tgtccacagc aggactgtta ctcaggtagg
421 aagaacaggg gtgatgcttt agttgtgggg tcccagctag gttaggcaac cagaagtgtt
481 gacatgttgc agtatggaag tgtgtcccct acactggcac actggtgacc tcaccttcac
541 tagcccactt ctagagggcc gagctcctgc cactggaccc tggagccctc cacccccacc
601 ccgtatgtcg ccccactgct gtttttagtg ttgatgtttt ggtgctgata attgccctgg
661 ggcacatgac cggtgc
//