LOCUS BG066825 480 bp mRNA linear EST 26-JAN-2001 DEFINITION H3047D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047D03 3', mRNA sequence. ACCESSION BG066825 VERSION BG066825.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 480) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3047D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..480 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047D03-3" /db_xref="taxon:10090" /clone="H3047D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 111 c 122 g 103 t ORIGIN 1 cccaaaagcc cagaatattt attacatgca aataaggaca tccacataca tagggctcaa 61 ttctgggtac cagaagcaac cttattctac ttactgacaa atgcacggac aaaggcaatc 121 tgaagccccc aggttataat aataatgaca ggaatgtaaa cacttcaagc aggtttgggt 181 agtaaaagag actttctggg gcggcctttt ggccttaagt atgtccagca gttcgggaca 241 aagttcctaa aatcggcctt aaagtatctt gaaaccccca aagcattccg gaggggcagg 301 gtacagcccc tgggtcagcg ggcccagttt ggctgtggga caaagaaggg ccttcaggac 361 acgcagggag ataccattat gacagaagct gactttaagg agctgggagc atttgcccag 421 agcaggcatg agggcttgga aatgagggcc ggtcattcca cacctctcca gttccagaat //