LOCUS BG066771 721 bp mRNA linear EST 12-MAY-2010
DEFINITION H3046G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3046G04 3', mRNA sequence.
ACCESSION BG066771
VERSION BG066771.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 721)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066771.1.
Other_ESTs: H3046G04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3046 row: G column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..721
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3046G04-3"
/db_xref="taxon:10090"
/clone="H3046G04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 256 a 115 c 113 g 237 t
ORIGIN
1 ccatagatca ggataggcat gtcctcaaag acaagaagct aactacccac agtaagataa
61 gaaaacaact taacttgcta tataagactc tcagagaact catattataa tgacagttta
121 aaggaataaa aaatgaatcc aggagttttg gtattcatgt gttaagatac ttttaatcaa
181 aagattattt tcaactaaaa agatagttct tcttcttgaa gaaaaataaa aacaatgaat
241 gtgtaaggag ggaaaaataa ttactcatag tcccattatc cccagaatag caataatgca
301 tgaagcgttt ccatatctta gtatattttt tgtttgcatt tgacgttcta ttttaaatgt
361 attgttatag taggcaaaat tttcttatta ttagacacat aggcataatt tcacttttta
421 tagcattccc tcaagaggat ataccgttaa ctactcctct ttggttgttt atttaaatta
481 tttacaattt gcattcctat aaataaacct tgaggaatag ctttacacat gactattttt
541 ccatagttag aattatttac ttagaaaaca tctgaggaag tagaattcct tgggcaaaga
601 agtaaaatac ttcactggct tgcatatagt attctaactt caccactgta agcctgagac
661 ctcagcccta ggaaatgatt tgaataagac aagcaagcaa gggatttgtg ggtctttctg
721 c
//