LOCUS BG066676 575 bp mRNA linear EST 12-MAY-2010 DEFINITION H3044G09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3044G09 3', mRNA sequence. ACCESSION BG066676 VERSION BG066676.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 575) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066676.1. Other_ESTs: H3044G09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3044 row: G column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..575 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3044G09-3" /db_xref="taxon:10090" /clone="H3044G09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 139 c 143 g 146 t ORIGIN 1 acaatgtggc tgaggcatga gcctcttcct ccgccatagc ccaggctctt cttttgactc 61 tggaaactcc agggcggggc tgatggccat gatgaagggc gagaccctca cattggcctg 121 gatgagaccc cagtgcctgg cagaggctct gctcatttac atttttatgt ctttatttgc 181 ctctccatgg ataattttaa taactgaaag tgttccaggc aagtggagat ttatatttaa 241 actcacattg gcatcaccta acaagatttg agtctccctt gaaactatta gaaatacatt 301 tcaagaccat ttgccagaat tatgaacttg agctgagaaa attccagaga aattagccct 361 cccgggagag ccagactgcc gtgggagtgg aggacacacc aagggttcag gatgcagtgg 421 gacctcaggc cactggggca cagccacctc ctgtccctgg ttcttgtcaa gtctgagggt 481 tggcagagtg ggaagcaacc atgcttctaa ctctgaggcc agaaaatgtt ttctgccaag 541 gacaaccggc taagtagtca ttgtcacagt tcagc //