LOCUS BG066626 746 bp mRNA linear EST 12-MAY-2010 DEFINITION H3044C06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3044C06 3', mRNA sequence. ACCESSION BG066626 VERSION BG066626.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 746) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066626.1. Other_ESTs: H3044C06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3044 row: C column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..746 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3044C06-3" /db_xref="taxon:10090" /clone="H3044C06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 173 a 213 c 184 g 176 t ORIGIN 1 cttcaagaca gggtttctct gtgtagtcct ggctgtcctg gaactaactc tgtagaccag 61 gctgacctcg aactcagatg gatttcttag taactgccca cacccagctg ggcaaggctc 121 tgcagatctg aggacataac tctccctggt gccatctgct ccaacatttg acaagagcac 181 ttaacgaggc cagtgtctga ggccgccagg gaggtgcggg ggtacccaca tccccacgag 241 gcctcatctg cactatcaac tgcatgaggg tgagtcagag tgatgggctg ctgctgctgc 301 tgaggtgtgg ctctcaacag gcactcagga gcctctctct cccaggcctc catgactgca 361 ttacatggtt ggaatccaga tgactggtca atgccactag ccaaaggctt tctcctcagc 421 aaatatgaag cccagacttt caaaaatcct gttctgaaat aatctgcccc aagggccact 481 taagtcaggt gacagtgtca gagaacagga ctcccaggac cccaggcaac tgcccttcta 541 cacttctgag aagccactgc cttctttgtg attcaaacca aggtctcacc atcactccag 601 acatgaactg tgcatttgtt cttctgctag gtcataacag cagcttaaag cgcttctttc 661 tcactgcggc ggggacaggt gaggagatgc tgctgctagg gtgctttccc ccaactttac 721 agccatgctg gggcagatgg gctcac //