LOCUS BG066566 771 bp mRNA linear EST 12-MAY-2010
DEFINITION H3045D11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3045D11 3', mRNA sequence.
ACCESSION BG066566
VERSION BG066566.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 771)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066566.1.
Other_ESTs: H3045D11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3045 row: D column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..771
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3045D11-3"
/db_xref="taxon:10090"
/clone="H3045D11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 189 a 175 c 200 g 207 t
ORIGIN
1 gtttcttggg gtgatctgga ataactgagg gtctggtaac agaccagttc tgcacagaag
61 tcgaagcagt agcaggactg gccctattga cgctggtgtt tccgttaggg cttgacacag
121 aactacttgg tgtgtctttc actggcgtgg tgccattcaa gtgtggcgtg ttcttcatca
181 catgggaagg cagctggggt ccaataaacc ctggagccac ctgcttgttg ttgactaccc
241 gctgactgac tcctgggcgg ggagaggatt ggccggggct atgggcagga tgagcagact
301 cccctccatt tttcacatca tgggacctga tataaaagag cacgtaagct tgctggttaa
361 gcactgctct gatatcactg gtggacacga tggagtcatt catctgatac cagaggccat
421 tgctagcctt gatgtagcaa aagtagtggc cagcatgaca attaaaacca gtgtgcacca
481 gcacagcata caaaacataa ataattggct ctccattggg ctgagacata tagggccgga
541 tatcaaggta ctcaggatat ttcacatcct tagcaatctt tccaccggtg aagttggcaa
601 agcgcttcag tgagatggta agaacattag aggacctatg gattgtgaat ctccttgaag
661 ctggaaccat ttttttgcac ttgctgcact tgtaggagtt ttctccatcc agttgttctg
721 gcttcacaaa ctgctctaac gccttggtaa cactctgtgc agccttaatc t
//