LOCUS BG066546 737 bp mRNA linear EST 12-MAY-2010 DEFINITION H3045B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3045B11 3', mRNA sequence. ACCESSION BG066546 VERSION BG066546.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 737) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066546.1. Other_ESTs: H3045B11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3045 row: B column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..737 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3045B11-3" /db_xref="taxon:10090" /clone="H3045B11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 239 a 185 c 161 g 152 t ORIGIN 1 aaaaaagttc ttttttggca gggggacttg gggggatgat aataatcctg tgttcattcc 61 accaaatttt agcaaaatcc aaatgaccta ggggcctagg aagatggctc agtggctgac 121 cgtccagaag accagggttc tagtcccagt acctacacag tggctcaaaa ccatacgtag 181 ctctgtgaga ttcctggggg tctgacaccc tcttctggca cctgtaggca ccaagcaaga 241 atgtagtgca cagacatgca tgcaggcaaa acatccatac acattaaata aataaataag 301 acttaaaaaa aaaatccaaa tgagagaaat cttgactacc caactaccta tcagaattct 361 caaagtcatg tgacagatac aaatatgaag tatgggactg agtggtacaa cctggcagga 421 agatcacaag ttcaaggtca gcctaggctg gaactcaggg ccaacctagt caacttgatg 481 tgaaaagatg agaccctgtt tcagaaagat gttaatgagc agggtcagtg aggcgctccg 541 tgggtaaagg ctcttgctcc caactctgac aatttgagtc tgtcagacaa gtcccagagc 601 ccacatgacg gaaggagaga ctcgatccct gcagactgcc ctctgacctg cactgctcac 661 acacacacac acacacacac acacacacac acacacacac acacgtaaca ccgtgaattc 721 agccacatgc ccgagtt //