LOCUS BG066494 837 bp mRNA linear EST 12-MAY-2010 DEFINITION H3043F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3043F04 3', mRNA sequence. ACCESSION BG066494 VERSION BG066494.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 837) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066494.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3043 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..837 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3043F04-3" /db_xref="taxon:10090" /clone="H3043F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 230 a 199 c 188 g 220 t ORIGIN 1 cgttttttcg agacagggtt tctggaactc actctgtaga ccaagctggc ctcaaactca 61 gaaatccgcc tgcctctgca tcccaagtgc tgggattaaa ggtgtaagac accaccgccc 121 ggcaacagtc attgaattga gagtgaacct ctcgccccat acaggcatgt gtgtccccat 181 ggccaaaact tggctatttt tgctattcta aggccctttc acatatgtgc tcgttttaga 241 gccacatagg atcagggaaa gagctgcccc ccatctccag aggacggctg ggttccacca 301 accctcccct gtcctctatt ccatttcact gttagattgt caaggttggc ctgagtccta 361 aacagacatt ttacagcagt agcaagggaa acagccatga gttagtatga ctgttgagca 421 cccactgatt ggtctggtca gaatggtcag gggaatgctg ggggaataat taagggagag 481 cagaaacagg gctagggagc acggagatct ctcaggtaca gggcagaacc atctataact 541 ggacactact ttttattctt aaacattttg aaagcttttc tttaatgaaa tttttactga 601 gttttcaagt gttctggtgg ttaaaacaaa acaaatattg acaaaggcta tggttcaaaa 661 actaaaaact caaaatgaca tgattctact tataacccta gtgtttcctg gaggcctggg 721 aaatggcagc catgagaagt gccaggtgct cctgcctcta ccactgcact cctgcagact 781 acctttcacc ataacgactt cgatgctgag agaaatgctg tgactagcag ccatttt //