LOCUS BG065942 574 bp mRNA linear EST 12-MAY-2010 DEFINITION H3037B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3037B03 3', mRNA sequence. ACCESSION BG065942 VERSION BG065942.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 574) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065942.1. Other_ESTs: H3037B03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3037 row: B column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..574 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3037B03-3" /db_xref="taxon:10090" /clone="H3037B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 89 c 98 g 204 t ORIGIN 1 gtagaatctt tcagctttat tggagttagt tacaggacaa taggaaagga gtttttgagc 61 agatcacaag agaatcagag tcacttgtta tcctaacaag catttgagaa gaattatgat 121 tactggatac tcgaagcaca cttcacatct gtaagcatat ttataggata aagaatatgc 181 tgtctgggtg gctcagtttg ccaggtactc ttttttaagt ataacttttc ttaactggat 241 catttaaatt aaatatacag aatgttatca gttttattag atattttgag gattgcatta 301 aagaccttaa atagttccct tttgaaatgg attacttctg tctcaataaa agtgttatac 361 aatactatct tctgacttcc caacttgtct tcatcttgta acgtttgagg atgaacaagt 421 aaaaaggtgt catgaaaagc tttctacatt ttcacatttt cagagtttct cacagcataa 481 atagttgaca ttacctaaga tttgaacatt gcatgaatat tttcacaaga ttttcataat 541 catactagtt ctctagtgtg gagcctcaag tgtt //