LOCUS BG065825 496 bp mRNA linear EST 12-MAY-2010 DEFINITION H3035F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3035F10 3', mRNA sequence. ACCESSION BG065825 VERSION BG065825.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 496) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065825.1. Other_ESTs: H3035F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3035 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..496 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3035F10-3" /db_xref="taxon:10090" /clone="H3035F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 165 a 75 c 131 g 125 t ORIGIN 1 gatttcaaaa tatagattta tttattaagg attatttagt ttctgcataa aaatcaccaa 61 ttgttcctca ttgggctttt tgggtttttt ttttttttaa acatacatca attctttcag 121 ggcaggttta ccccaatact ttaaaaaata cagctccaga aggacggtta aggcagcaag 181 gcagtgtctt gagtctccgc agctggaggg ctcttatgag aggcagtttc ttaggttgga 241 agagccaacc cccaagaaaa taattatgca gaagaatgca aatacagtca catatgggtc 301 acctgtgtgc gcctagtcag ttggttgaaa aggcttgctg gtacatttca ggggaaaaaa 361 aaacccaaaa aacattatct gacaaaagac agaggggggg tgagaagaga gagagagaga 421 gtgagacaga gaggagagag agagaggaga gagagagaga gaacgagaac agggctggtt 481 atgccagatg gatgtg //