LOCUS BG065822 560 bp mRNA linear EST 12-MAY-2010 DEFINITION H3035F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3035F07 3', mRNA sequence. ACCESSION BG065822 VERSION BG065822.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 560) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065822.1. Other_ESTs: H3035F07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3035 row: F column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..560 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3035F07-3" /db_xref="taxon:10090" /clone="H3035F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 120 a 138 c 139 g 163 t ORIGIN 1 attattttat tttatttctc attggtattg ggatggaagc taggccctgt gcttgctggg 61 caggccctgt gctgggaaga ccctgtgcta ggcagatgct tgcttgctgg gtaggcgctg 121 tgctggagag gcgctgtgcc actaagctat gtccttagcc ctctttttac tttttatttt 181 gagacaggag ctctgcaaat tatccaggac agtcttgatc tttcggccct cctgcttcga 241 cctccttaag tagcaaggat tacagacttc tataccaccc tttcaccctt tctctttttt 301 atgtctgagt gtttcctatt actgtgataa accaccttga ccaaaagcaa ctaggggaag 361 gaaatggttt atgtcacctt acagctgata agttcttcat ccaggggcag gaactcaagg 421 cagcaacctg gaggcaggag cagaggccat gggggagtgc tgcttactgg cttgcttcca 481 tgacttgctc agcctgtttt cctatagagc ccaggaccac cagcccagag acagaatcac 541 ccgcaatgag ctgggccttg //