LOCUS BG065749 867 bp mRNA linear EST 12-MAY-2010 DEFINITION H3034G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3034G08 3', mRNA sequence. ACCESSION BG065749 VERSION BG065749.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 867) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065749.1. Other_ESTs: H3034G08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3034 row: G column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..867 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3034G08-3" /db_xref="taxon:10090" /clone="H3034G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 206 a 223 c 217 g 221 t ORIGIN 1 aatatatctt tgtatgtgtg agtgtttttg cctgtgtata tgcctgtgca ccacatgaat 61 gcagtgtctt tggaggtcag aagagggcat tgcatctcct gggattggag ttacagacgg 121 ttgggagctg ccatgtgggt gttagggatt gaacctgggt cctctggaaa agcatacagt 181 gctcttaact gctgagtcgc atctccctag cccactggtt tactcttggt cacagctcac 241 acttcttccc ctgttttctt tttgtccctc aggccagcag tttccaagct ggggggattt 301 ccttctcacc acagcacctc tgagagcaca tggcaatgac cagagatgct gttgtgcatg 361 cctggagagg cacaggatgg tagcccaagc atgagacagg tgaaagtgga ggtagctgta 421 aagatcctgc aggacccaca acccagccat cttcagcctc aatggcaccc aggctgagat 481 ccttaggtcc aggtcacacc atcctgataa catgctggac aatactgtca ctttcagggg 541 ccacaagtga tagccatgcc ttcacaaggt cagcctccaa cttttgttcc acagtgctca 601 actacctgga agtcaaagtg agactaggct tacaggcaag acgtacctgt gagaatgcag 661 gggaactgca gggtgtagtt tgatatccca gaagctcatg gggccgtgtt atatttggtc 721 atccactagc atcacccttc cagcagggga ctcctgcccc tagtcactgc acccttgctc 781 tgaacaggaa ccaggagaag aatgcacacc gtcttggttt tgtatcctgt tgctgccatc 841 aaataccctg acacaaagca tcttaga //