LOCUS BG065705 880 bp mRNA linear EST 26-JAN-2001
DEFINITION H3034C08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3034C08 3', mRNA sequence.
ACCESSION BG065705
VERSION BG065705.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 880)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3034C08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3034 row: C column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..880
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3034C08-3"
/db_xref="taxon:10090"
/clone="H3034C08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 261 a 182 c 189 g 248 t
ORIGIN
1 gtgtggaatc tgaatgtaat tttacaactc aagcatcaga ctttttatgc agaggacaat
61 aaggaagttg ggtgacatat tcgcaaggta caattgaggt aaccggaatc ttacataaaa
121 cagaggaatg caaacacaaa aggtctaacg ggaaccaccc aggatagaat acattgatat
181 cagctgggat caacaaaggc tccacctaaa attcactaat cttagaagcc agggtcaagg
241 gcttcacgtc cttgccatag ttcctatttt agtctcttgt atagtccacc ttccccttag
301 gccattgtaa atatgtgtat gggtgtactc tgctatagta tctattctgg tttctccttt
361 ggtctgaaac ttccttcttc ctaagcgatg gtaaattcct gtgtaaggga gtgacttagc
421 ttttacaatc ttgctgaatt ctaaaccctt gatcttggtc aaggatgccc ttggactgtt
481 ggaacactgg cggaaggctt tagccatgtc agagcactta taggaaaata ctgaaagaga
541 gcacgtggat ccatactcta gactagcgta gtactggaac tcgggaatga aaaattaatg
601 tatgggtaga gaacactagc ctccaggagg agagcttcct tgaaactctt ttgcctcatg
661 agtgactttt aagcctttcg gcttgtccag ttgactcgac cagagagcgt acgatttctc
721 cctttctatt tttatttttc agccgaagtt ccaggatgtc ccgcctcatc gtagctagag
781 ctcaggagca ctttaaagat gagtggaaca ccacaattac tagtaaaaca atgccaaaaa
841 taataacaag taggataata tattgaaccc aatccagggg
//