LOCUS BG065689 730 bp mRNA linear EST 12-MAY-2010 DEFINITION H3034B01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3034B01 3', mRNA sequence. ACCESSION BG065689 VERSION BG065689.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 730) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065689.1. Other_ESTs: H3034B01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3034 row: B column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..730 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3034B01-3" /db_xref="taxon:10090" /clone="H3034B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 210 a 166 c 195 g 159 t ORIGIN 1 aatgtctgag aacattttta ttaaggacag aatggggtca cagcatctta gagagcctat 61 tctctgccat tgtgggaaga gcaggacgag acccgggcac ttcaaggagc cagggggatg 121 tggattcctg agccagcttg ggtcaggcgt gggtggctgc tccacaggtc tccctgccac 181 cttgctcaaa gcctgctcag ggcagggctg gacaggacaa ggcatgcaag cccagaggga 241 caccactgac tccaaggcag ggcaggccat ggttgtcaac aatggggctg agagtccctg 301 agaggggctg ctggcataag agtaccctcc catagtgagc agagagcagt gacgggtgac 361 aggcaggtag agctaagcaa tgagacagct ggactatctc tagattcaga aaagcaacat 421 gaacaaaatg gtggtttgct tcgacaattc tgacagagtt tggcatttta ttacaaatct 481 gaatatgagt gaaataaaat ccattgtcac ttttctgcca atgaagagac agatgtagaa 541 acacaggccc agggctctga ccatcctggg catatggaag tctccagaaa cctgccccat 601 gcataacttg aactaaagca tagttcattt atgcatcaca ggagccaaac acttggaaaa 661 ctaaatagtg ttacactgcc tgagaggaca tgtcttactt cttttatagg caaaacccag 721 agctgtctcc //