LOCUS BG065584 641 bp mRNA linear EST 12-MAY-2010 DEFINITION H3032H08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3032H08 3', mRNA sequence. ACCESSION BG065584 VERSION BG065584.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 641) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065584.1. Other_ESTs: H3032H08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3032 row: H column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..641 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3032H08-3" /db_xref="taxon:10090" /clone="H3032H08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 174 c 153 g 160 t ORIGIN 1 cactagcaaa tctggtactt tattaagaca ttttaggctt tcctgcattt tcaaaaacag 61 gaatgctctg ttgactgaac acatcaaaag ggctttgcag aatccttggg gaccagccac 121 aggaggtgtc acatgattct gcagtctcag agcatggcag ctagtttttg caatcacagg 181 aagcaggcaa ccggctctcc atctgcctag gggagggtgt ccttcattga atgactgcct 241 taaagtcaca gctcaattcc agtgtgtaga ctcagtctct ctaggactgg gaacaaaggc 301 agctgccagg tgcaacagtt tatgaggctc cgccttttat atatgccacg ggagcccagt 361 ctctggaagc ccaagatgga ccaaccactg ctgtgggctg atggccttag cagccacata 421 atcctccctt tgatgtccag ggtcagtgca gtcctggccc caccagggct aggagaaggt 481 atatgagaat ctcactgtga tctgtcctct ttagaacagc atttcagtca ccacctgggc 541 cgatgctctg tccatgtgtt ccttcaccag ctcaaagcac ctgtgggcta gctgctgcag 601 cctggcgtcc tcactcacca tcagctcccc atacaggtat a //