LOCUS BG065563 614 bp mRNA linear EST 26-JAN-2001 DEFINITION H3032F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3032F10 3', mRNA sequence. ACCESSION BG065563 VERSION BG065563.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 614) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3032F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3032 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..614 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3032F10-3" /db_xref="taxon:10090" /clone="H3032F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 171 c 141 g 176 t ORIGIN 1 aaaaaacttt ttattgattc tttgtgaatt tcatatcatg taccacactc ccccttatct 61 tcccattcct ttgtatctgc cctctgccct tgcaacctcc cccaaaataa aataagatac 121 aacaatcttg ttgtggaaac tgcagtgtgt tacagtgtgt cccacagtac acccttttgc 181 ccacacgtcg ttactttcaa acattcattg caatgagtca ttggtctgtt tcaaggcctc 241 tggcttctgc tacaccaatg gatcctcact ggggctcttt ttggatatcc tgctgttgcc 301 ctgtgtcagg gacatcctgc agctttgtat ctgagggact agtgctttca ttcgagtgct 361 ccagtgggtc atagatgggg tagatgttgg ggcccggcaa ctcaaagccc tggatctggg 421 acttagctga gttggtcagc ccactagctc tcctgcacct gcaccaccag ggtgagctct 481 ccagcactgc tcagctaggt cagctggcaa ggggcagact caactcccct gctctcctgt 541 ctgtgggacc agctcacctg tacttttgct accaggccgg atctactgtg ctgcagacag 601 ggaggggtgg ggca //