LOCUS BG065481 591 bp mRNA linear EST 12-MAY-2010 DEFINITION H3031F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3031F06 3', mRNA sequence. ACCESSION BG065481 VERSION BG065481.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 591) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065481.1. Other_ESTs: H3031F06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3031 row: F column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..591 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3031F06-3" /db_xref="taxon:10090" /clone="H3031F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 149 c 133 g 155 t ORIGIN 1 gagacaggat ttctctgtgt agccctgggt tctggaaccc actctataga ccaggctggc 61 cctgaactca gaaatccgcc tgcctctgcc tcccaagtgc tgggattaaa ggcatgtgcc 121 accactgcct ggcttatctc tacatttcta aaatgagggt ggggaaaaga aacactgtgg 181 gaatataaaa taacctttat cagaaagatg aagttttcat ccttcaactt ttaaaacatt 241 tccatgtccg tgtgcctgct tgtttgtctg cacaccatgt gcaggtgcct gtggggacca 301 gaagagcatg ctggatcagc tcaggagcag ctgatcttac aagcactgtg aacactggat 361 gtgggctctg ggagccctac ctgggccagc tgtgagagca gcagaccctc ttacctgctg 421 ctgagcatct ctccagcccc ctgaccttta actcactgag tttttccaat gttatgctgt 481 ccttaacatc agagattgct aatattttct gcattgccat gaggaacata gaaggatctt 541 agtgcaattt caaggatcac tgcattagaa aacagaaccc tccaaaaaac c //