LOCUS BG065310 749 bp mRNA linear EST 12-MAY-2010
DEFINITION H3029F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3029F06 3', mRNA sequence.
ACCESSION BG065310
VERSION BG065310.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 749)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG065310.1.
Other_ESTs: H3029F06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3029 row: F column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..749
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3029F06-3"
/db_xref="taxon:10090"
/clone="H3029F06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 229 a 162 c 195 g 163 t
ORIGIN
1 gttcagccat gaatatttat tggtaggtac cattgtaggt taacacaggc gactgactca
61 ttcatagaac ctgccacagg gggcttacag tctatgggac tggtgagaga aaacagtgtg
121 ggagatacaa ggccagagtt gtttccaaaa tattaatcta aagatgaagc aaagaccagc
181 aagatcctgg aggtgggagg agtgaagggg gaagtctgtt gaggtagtat gatgatggga
241 tttaactgaa ccctggatgc agggagggag gaaacagacc actctcttct agcatggtga
301 agcttgaata ttgagcttgt gagaaatcag ttgttctgtg aggataatgc ctaaaatgga
361 ctagggacaa gcaggaagaa agtcttagac cagggtcttc ctcctcacac atgccccccc
421 ctctcaagcc ccagcctccc aagaacttcc tgtactgaaa cgtcacagga tctgaaactg
481 atggacggct atccccagtc actgactcaa ccatcatggg gacttcaaag tgtcatttct
541 cagttcctta agaagtcaca acctagcccg gtggtaattc caactccagg ctgaaccatg
601 agagacgcag agtcctctag gaagaccctg cttatgacag gaatacgaca gagctacaaa
661 cggacaaaca aaatgaaagg gggtgaatct gttcacttac agtgctcaac ggggacaggt
721 cttgacaaaa cccagcatga gagagaggt
//