LOCUS BG065287 761 bp mRNA linear EST 12-MAY-2010
DEFINITION H3029D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3029D03 3', mRNA sequence.
ACCESSION BG065287
VERSION BG065287.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 761)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG065287.1.
Other_ESTs: H3029D03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3029 row: D column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..761
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3029D03-3"
/db_xref="taxon:10090"
/clone="H3029D03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 238 a 157 c 175 g 191 t
ORIGIN
1 cctttcgcaa cagaatctat actggtctgg ctggcctaga actcactata tagaccagac
61 tggcctctaa ttaagattgg tctacttctg cctcctagat gctgtaatta aaggtgtatt
121 acactatacc tggctaacat aggtaatttt gaaggtccag tctctacaaa agaaggagcc
181 agatatggtg acaaacactt acaatcctag agactgggag atagaggcaa gagatcaaga
241 attcagcttg gcagggctgg agagatggca cagcagttaa gagcattgac tgctcttcta
301 aaggccccca gttcaattcc cagaaaccac atggtggctc acaaccatct gtaatgagat
361 tcaatgccct cttctggtat gtctgaagac tgctacagat gaaaaaaaaa attcttttca
421 acttgtctat acggcaagtt gaggctagcc tggtatcaga gactaagtct ttagaacaac
481 aataaaatgg tgtatggcgg ggaaggggca gaaaaagaca tcagttcagt ggcacagcac
541 ttgcctggca tgtctaagct caagtcccag taccacaaaa ttagttttga aagaggatta
601 acacaggaga atatagtcaa gtgtttagga gtatttcacc tttggttgtg aactctttag
661 agggaagata gctctaggaa acacttctgt ccaaggctgc aattctccag cagaaacaag
721 ctacttggca caacacatgc tggaggcaca gatgggtgtc t
//