LOCUS BG065211 561 bp mRNA linear EST 17-DEC-2003 DEFINITION H3028E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3028E07 3', mRNA sequence. ACCESSION BG065211 VERSION BG065211.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 561) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT On Jan 26, 2001 this sequence version replaced BG065211.1. Other_ESTs: H3028E07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3028 row: E column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..561 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3028E07-3" /db_xref="taxon:10090" /clone="H3028E07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 142 c 137 g 123 t ORIGIN 1 acgcaaactt gtctttatct ttaatacatg gaaaattatt tgtattcaga atcactttaa 61 aatagatttc ttgggatata tttatctgta aagctccttt atatacctcc atacctggga 121 gtatataagt gactctggtg cagggggctg tgggggagtt caagaagcag aagggggaca 181 cgccatggct cccatctgca gatgggaatc cagatacaaa tgcttagtcc ttctgtttcc 241 aacgagctgg cagagaggtg caatgacaag gaacacggcc cagctgctga gactaggaac 301 ggcatggggt tgttgttgaa acgagtaggg atcatggagc agcagctttt accagctcgt 361 gggcagccac actggcccag ggtcctcaat acaggctctc cctacagacc agtgagtcgc 421 attcaggctg cactgcaatg gcacccagat cgaatatgta ggagtccccc accacacaca 481 cacacacaca cacacacaca cacacacaca cacacaccag ggaatcccag ctgatgggga 541 aagccagctt gggccagtct c //