LOCUS BG065179 699 bp mRNA linear EST 12-MAY-2010
DEFINITION H3028B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3028B10 3', mRNA sequence.
ACCESSION BG065179
VERSION BG065179.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 699)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG065179.1.
Other_ESTs: H3028B10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3028 row: B column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..699
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3028B10-3"
/db_xref="taxon:10090"
/clone="H3028B10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 172 c 154 g 196 t
ORIGIN
1 ctgtagtagc tcaagtttat tgactatagt acatggtaag cacagagaag aagcaatcgc
61 ttaagaacac caaaaaccct tacatagcaa aggttaagaa ataaattagg actcctagtg
121 ggctgtgcct ggggctggca gggcatcatt cacttctatc cttgggctcc cggtagcgcc
181 actggatgta atcaaaaatg tcctgctcac tgtccacagg aaggggctcc ccagcgactc
241 cagtgacccc cagggggcgg atggtgtact cattgattgt gaagcccttt tccagggcgt
301 gcgctctcat gttcttatta aagatgtcac tcccagtgaa gtagagaaca ccacagtagt
361 actgatcttt ggggatcaac ctgatatcga ttctcctgtg tggatattcc tttccatcct
421 tctcgctggg aagctggcaa acacccatga actttgtttc accctttgac agagtatctg
481 taatgaaatg cactttttgt aactgttcca caacacgatg taacaacttt ggctgtttgc
541 tggattctga cgtgaagttt gggtgggtca gcaggacatc catgtctcca ctcgactctg
601 cgcctcttct gaaactgcca cagactgtag caatgtactc agagtccact tttttaattt
661 cattaagtac aatatcctgc atctgcagca tttcctcac
//