LOCUS BG065178 587 bp mRNA linear EST 26-JAN-2001
DEFINITION H3028B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3028B09 3', mRNA sequence.
ACCESSION BG065178
VERSION BG065178.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 587)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3028B09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3028 row: B column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..587
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3028B09-3"
/db_xref="taxon:10090"
/clone="H3028B09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 260 a 105 c 107 g 115 t
ORIGIN
1 cagaagaaca cactgaatct ttatttcccc tttaaccaag tacaaacaaa aaatgcaaaa
61 acacgcttag ggttgggagt tgggaatcca aaaaatccaa aaaacagaaa agcagggaaa
121 aagcaaaaac aagcattaaa aggattttta aaaagtaaat gccggtttaa aaaaaaatgt
181 ggtaacaaaa gccagaatta gatcccaata aaacaaggca caatgtttaa ggcaaaattt
241 atgaagaaag aatataaagt taaaaaaaga ccaaatttta aaaaaccctt ggggggaaga
301 ggcacaagaa tttaaaatag cttaaacatc cttttaagaa tattagccaa aaaaaagaaa
361 aaaaaaccgg atacaatagg actaaatttt tcccagaaaa aaaaaccccc acgggggaac
421 caaattccgg ggcaacattt aaattaggga aacccttata cggatattcc caatacggac
481 tttaggtatg gaaaattaag aggccagggc atacgaaatg accggcccaa agtaaagggg
541 aacacctttc cagcaccccc gggggttttg gaacccccca attttaa
//