LOCUS BG064988 655 bp mRNA linear EST 12-MAY-2010 DEFINITION H3026B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3026B04 3', mRNA sequence. ACCESSION BG064988 VERSION BG064988.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 655) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064988.1. Other_ESTs: H3026B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3026 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..655 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3026B04-3" /db_xref="taxon:10090" /clone="H3026B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 146 c 119 g 211 t ORIGIN 1 caggttcaat atagcaattt aataataacc tcagaaaatc tcaaagctta aagtatacat 61 acataagcat gtggtatcca cttggtacca cctgagtaac aacagtatgt agaccacttc 121 ttgatgaccc tacagtctgg aagcaacaca tctgtccagg cagctaccat tccagctcca 181 cagggtactg cccagtgagg caagctgtac agtgtcccgt cttttcaaaa tattccaaac 241 catttccatt ttcttgaatt gcaatatcat gttttttcac tttctgcttc ttaaatttta 301 tttcttgttg cacagatgaa accagtcctt ccactgacag atacacaacg ctgtttgctc 361 cgaggtactc tgcaagacaa tcaaattctg gcttattggc tatgagctct tcttttgtag 421 gaatgtttat tcccatgaag cacggatatt ttattggtgg tgaaggactc gaatgtgcac 481 ctcttttgca ccggattctt tgaggagttt tatgatgggt gagatagtat tgcccctcac 541 aatcgaatca tctatgagaa cgattctttt gcctttaaag ttgtcagaca acacgccaaa 601 cttctttgca acaccgagtt gtcttaacct catgtttggt tgaatgaagg ttctt //