LOCUS BG064961 495 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025G11 3', mRNA sequence. ACCESSION BG064961 VERSION BG064961.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 495) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064961.1. Other_ESTs: H3025G11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3025 row: G column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..495 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025G11-3" /db_xref="taxon:10090" /clone="H3025G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 102 c 104 g 135 t ORIGIN 1 caatatagaa tatttatgag tcaagacaat caaccgtgta caaaaaaaat ttacatgcaa 61 gagaaacaag gcaatgtagc acacctctag aaaaataatt aaaagttttg ctgcatttac 121 aggtaagtgc cacactgacg gatttacaac acagtaattt actgcaatca caggggagtt 181 ccatcaagaa acaaagtcct tacactccag agcttttgga aggtattgtc ggaatgctta 241 attacacact gaagattcct acatccttgg gattgagctg acaaatcata aaggattttc 301 gtagcatata ttagataaaa agagtccatc atatttagac tgtcggttta gtgtctctgg 361 caggtgtgga gcctagaaga attgctggct tgacggtgat ctttctgtat cctgcaagcc 421 aggccttccc cgcagtaaca gcgctggaat atctccagcc cgtgggagcc tttccgtttg 481 tgcttggtgc cacct //