LOCUS BG064956 744 bp mRNA linear EST 12-MAY-2010
DEFINITION H3025G06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3025G06 3', mRNA sequence.
ACCESSION BG064956
VERSION BG064956.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 744)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064956.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3025 row: G column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..744
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3025G06-3"
/db_xref="taxon:10090"
/clone="H3025G06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 219 a 155 c 126 g 244 t
ORIGIN
1 atcatagaaa acatgtatca tttattacat ggacttagaa atcaacacaa atcaatgtca
61 ggcacttttt aagtacacac tatgagaaat gtctaccaag ggtatgcaca atgagcaggc
121 aacaccacag agactcattc atccgtcttt aggaacaata cagagttaga aaattaaaca
181 gatgaaatat tgcataatta gcccaactgt atcctgtcac acttcttccc aatttcaaac
241 cactttaggg ttaaatccaa gcacttactt tttaagcatc cagagcgcta catctcactc
301 tttcccttac tccactatca tggtcttaaa gagtcacacc tgctgtgagg aatgtggcac
361 agactttgtc agaagcaatc tgaaactatg tttcctatta catctgattc tacagaaaca
421 catgatggtt gattgctggc tttttcttcc ttcctttcct ctgtggcaat ctttcgttat
481 gtagtcctgg tggctgatat tatttagctc cggttgactt tgtactcccg gcaattctcc
541 tgtctcagct tcctgagtgc tgggattaca gtcttaacac cactacatat ggcttatttt
601 gctgtctttt aaagcctgtt ttagcaggtt tattgtgtaa aaatactagg tctcaaatta
661 caaattcctt tagacattta gtgttgattt ttgctattta agcaaacata aggaaaaata
721 gagtgtggga aatcattgta ggat
//