LOCUS BG064935 776 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025E07 3', mRNA sequence. ACCESSION BG064935 VERSION BG064935.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 776) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064935.1. Other_ESTs: H3025E07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3025 row: E column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..776 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025E07-3" /db_xref="taxon:10090" /clone="H3025E07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 213 a 200 c 187 g 176 t ORIGIN 1 gcacaattct gtgataaaaa caaaaactgg aatacaaggt tatgaggttt ctgaatggga 61 aaagctgacc ccatcaactg aaataaaaag attctcatac acaagagagg aactcgggga 121 gaacagggta aatgtggaaa ggcagggcta aacgcatcca agggacttgt tatctcagaa 181 ctcttcaaac acaaaattag gattcttgac tctcaaaagg ctgaagagac tgagcttcag 241 ggctacaggg aaagaaaccg ccagaaacaa accaccttct ccctagggat ctaatacagg 301 gtcttctttt gacccgagag aatacaaagc tcttccggcc tcttctagcc gctgtcggta 361 ttcgagcttc tcgtagttgg cgggatttac gcctttaaag ccgtacataa ctccccacca 421 gcagccagca atggcggccg tggaatcact gtctccaccg tggaagaagg ctttgtgtgc 481 gagctccttc caggaatccc ccgcggccag gagggcatcg taggcaatca tgggggcatc 541 gtgtccgctg ctgccacccc agcccgagta gctcacgtcg atgtagaact gatccctttc 601 cttcacacca aagggctgcg ggaagacggg agccgagttg ccgtccaaaa ttcctctaag 661 ctccaggtac ttttcccatt ctttctcgaa gtaggaccag tgttgaagat tctccttcac 721 aaagtagcct gactgagtga tgtacttttt ggcttcaggc agcacctcca tttagc //