LOCUS BG064907 575 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025B10 3', mRNA sequence. ACCESSION BG064907 VERSION BG064907.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 575) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064907.1. Other_ESTs: H3025B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3025 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..575 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025B10-3" /db_xref="taxon:10090" /clone="H3025B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 141 c 139 g 169 t ORIGIN 1 gagtaaccct ggactagagt gatctgcttt attagataaa gataatgagt attatcttag 61 gttttgcaag cacaacaacc ataaaaatgg accatgactt ccttcccatg ttttctcggt 121 ccatatgctt agcagagcag tagaggatga aagcaggaca gctccggtcc cttcagtggt 181 tcatgggtag gtgtgacctc gtccctgtcc gtcccacttg gaggcatggc aacttggagc 241 agttcttgag gagttgctcg atagccacgt gacggacatg gagctctgac gccagagagt 301 ccttctcttg cacctggtgt gttaatcttc aaaaacctct tggattcgct gttggagctt 361 cgcactcagt tgtactactt cagcaagagt catcgaattc aattcttcac ggctgtaatg 421 tggattctgg gtctggatag tcccctccgg accagagctt gtctcttctg ttttctgtgc 481 ctggctgtga ctgtgagtcc acaatgcatc cgcgtcttcc aataaaccca gccggaagtc 541 tgtattttct ctgtgctcca ggattctctg cgcat //