LOCUS BG064881 480 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024H07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024H07 3', mRNA sequence. ACCESSION BG064881 VERSION BG064881.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 480) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064881.1. Other_ESTs: H3024H07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: H column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..480 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024H07-3" /db_xref="taxon:10090" /clone="H3024H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 107 a 135 c 128 g 110 t ORIGIN 1 cttgccaaac tttattatga ttaaaattcc aaagacagta ctggctccac acaccttcgt 61 ggccctgtct ttgcctcgat tcctgggtgt ccttcaccac ccatcttcca aatgtcaagc 121 ttcatgagga ggaagagagc tctaggtctg gatgtgtcct cttgggaccc agagttggcc 181 cgggtgctga aagaaatcaa gatgaaggtc tccccgcctg actgcgatgg tctcgaaggc 241 aacgtgtgga gcagaaagag aagtcgaggt agtgaaaggg aatgaggcct tggagggaca 301 ctccacaact ccagcagcgt ccagcactag caactgcaga gtcaggaact ggagggctag 361 gggctcccaa ctgggcagca agtcggcgct ctgcagccag agctctcttc tcccggtcac 421 tgagggcagc aaaccgccgt tgttcctctt gctcttgttc ttcctgctcc ctctgcttcc //