LOCUS BG064868 740 bp mRNA linear EST 26-JAN-2001
DEFINITION H3024G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3024G04 3', mRNA sequence.
ACCESSION BG064868
VERSION BG064868.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 740)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3024G04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3024 row: G column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..740
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3024G04-3"
/db_xref="taxon:10090"
/clone="H3024G04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 229 a 115 c 176 g 220 t
ORIGIN
1 attgagaaag acatatagga tatgagattg gcttgaaacc aattttaggg ggttcgattc
61 cttcctttct tattttactt ttacataggt tggttcctcg aatgtgtgat atggtggagg
121 gcagccatga agtcattcta aatttgttga agcatacgat actgatatta cttctcgttt
181 tgaagcaaag gcctctcaaa ttataaagat catgatgaga acagctgtta gtgaaataaa
241 tgatcctata gaagagacag tgtttcatgt ggtgtaagca tctgggtagt ctgagtagcg
301 tcgtggtatt cctgaaaggc ccaggaaatg ttgagggaag aatgttatgt ttactcctac
361 gaatatgatg gcgaagtggg cttttgctca tgtgtcatct agggtgaagc ctgaaaataa
421 tgggaatcag tgaacaaatc ctgctatgat agcaaacact gctcccattg atagaacata
481 gtggaaatgg gctactacat agtatgtatc gtgaagcacg atgtcaaggg atgagttgga
541 taaaacaatt ccggttagac caccaactgt aaataagaaa ataaagccta aggctcatag
601 tatagctgga gatcatttaa tattacctcc gtgtagggtt gcaagtcagc taaatacttt
661 gacaccggta ggaattgcga taattatagt ggctgatgta aagtaagctc gtgtgtctac
721 atctaatcct actgtgaata
//