LOCUS BG064845 689 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024E03 3', mRNA sequence. ACCESSION BG064845 VERSION BG064845.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 689) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064845.1. Other_ESTs: H3024E03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: E column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..689 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024E03-3" /db_xref="taxon:10090" /clone="H3024E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 202 a 173 c 172 g 142 t ORIGIN 1 aaccttgaaa ctgtttattg gtttattaca ataaacaata tatacaatac atataacact 61 gaaggtgtca ccatgacagc atgagcccac agttgcatcg ttccctctca gatgcagtct 121 tcctcactga ggcgagacaa ctttgggagt gatacagcaa aaatataaag tctgttctct 181 gcaggttctg agtttctact tattccctac aagttaaaca cgcccctgtg acacaggtgg 241 ttggtccatt cacacagtcc tcataaaagt agggatgacc aagtcaatgg cacacacagg 301 agatgggaga gaggttgctt tatcccatct cactctccag gacattccag gacctcaaga 361 caaggccacc ccaaggcagg ggctccaatg gcagagcatg agggtgccat ggatgaccac 421 tctgggacca cagccaaccc taggcagcca tgggtcagtg ggtcgtccca gcgtgaagga 481 aggaatgagg tgtggaaaga cactacgaag gggcagggaa agaccatcac attctgtgtg 541 gagcccgagt cagccagccc aggagagctg agacccagca agctagcctc aaggccctca 601 ctcgaagcag aagttcatga aggttgatct gccacaggct aaaggcctca ctgaaagata 661 tctgagtcct tctgaagaaa gagccggca //