LOCUS BG064797 779 bp mRNA linear EST 12-MAY-2010 DEFINITION H3023H12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3023H12 3', mRNA sequence. ACCESSION BG064797 VERSION BG064797.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 779) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064797.1. Other_ESTs: H3023H12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3023 row: H column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..779 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3023H12-3" /db_xref="taxon:10090" /clone="H3023H12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 203 a 199 c 189 g 188 t ORIGIN 1 cactgttcaa ggttttattt ggcatgacac ttgggtggtt ggttccatca tccatatgca 61 gatcttacat ctcacataat attgcaatgc acactacaga cacatataat atataaaata 121 tcctgtacaa catttatgca caagatatgc atcatggacg tacacactgg agccaggtta 181 tacgacttaa ctgggaactc gtcggcctag gcatgtttgg tgcagggcag caatgcagca 241 ggctcacagg ggtaatcgaa gcctgcagtt ggcagtgtgt ctcagagaca gtgggactgt 301 cacactaacc aggtcaccac tacacaagta ccacttggca gcagggcaga gctgtgggga 361 gtgagatgtt tccccacacc atctcaacac cactgtacta accacagctc agacgagaag 421 ggtgtggtct gcctagaagc cctgctgcag cctggacagt gaagtgctag gacacgggga 481 agactttaga actgcagctc cttctggatt ccccagaggg tgtctgcgct cttcttcagg 541 cgggcctctt cctcaggagt cagtgtcacc ttcacaacat ccgagattcc attttgtccc 601 aggatacatg ggacactgag gaagacatcc tcattgattc catagagacc cttaatcatg 661 gtggaaatgg gatgcacccg cctaaggttc ttcattatgc tctcagccaa gtctgccaca 721 gagaggccaa tggcccagga tgtgtaactt tcagcttgat cactcgtagg cactgtcca //