LOCUS BG064735 773 bp mRNA linear EST 12-MAY-2010
DEFINITION H3023C07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3023C07 3', mRNA sequence.
ACCESSION BG064735
VERSION BG064735.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 773)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064735.1.
Other_ESTs: H3023C07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3023 row: C column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..773
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3023C07-3"
/db_xref="taxon:10090"
/clone="H3023C07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 222 a 185 c 181 g 185 t
ORIGIN
1 ctgatcagga ttttatttgt tgaacctcaa aaagcagaat tcagaaatgg ccacccacta
61 ctaggacact gagtggtaaa acactgtcac cgcccgtctt ttataaggtg cgttcacagg
121 cagacatcac cagagagcag atgtggaaac tctaggaggt acatgcgcac tcttgggagg
181 cgaaaggctt tctgagccca cttcgataga aacttacaat agattccctg taagaattct
241 cccggctgtc cccacttttt atttactaaa aatacagaat cgaaagcttc tccctatgtc
301 agtgctctcc aaaatgggta acagaagatt cagttcggaa agatacaaaa ggtgtttcat
361 cattctagca cagagcccac actgacacta ccttgtttgc tgatcaaaga ccctacagag
421 acctaaggaa tcttcaatac ataattcgtt tcaaagagag ccagtcagta gggcactgtg
481 acaggcagag gggcagaatg ctggcgaagg cacagagtaa agaatcccga gaaggttttg
541 gtgccatttc catttaatga cccagtagta tagcgagcga tctacaggac cggctgtgat
601 catgccacga tctttctacg gacctgctgc aaggacccac ggctgccagc tgacatcctc
661 aggccccagt actggatgtg cagatgcagc cccaatttcc gcaaagactt aaggctgggg
721 tgtataacgg aggtattttt tgccagacgg gagctctttg gtgaagactc ctt
//