LOCUS BG064699 506 bp mRNA linear EST 12-MAY-2010 DEFINITION H3022G02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3022G02 3', mRNA sequence. ACCESSION BG064699 VERSION BG064699.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 506) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064699.1. Other_ESTs: H3022G02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3022 row: G column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..506 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3022G02-3" /db_xref="taxon:10090" /clone="H3022G02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 149 c 107 g 124 t ORIGIN 1 tgcaaacata ccagcttctg tgcaaacact tctaatctcc gctccagtac tgtttggaca 61 caggcgggcc aacagctcaa atcgaatgtc tctttccaca ctcattgagc gagcgtgaat 121 cttaaagatg tgcgttcgac cctctaagtc aggtaagctg aactcaatct tcctgtccag 181 cctccccggt ctcatgagcg ctgggtccag agtgtcaggg cggttagtgg ccatgagcac 241 cttgatgttg ccgcgagatc aaagccatcc agctgattga tgagctccag catgttctct 301 gcacttcatt gtcaccccca gcgccatcgt caaaccgagc ccctccaatg gcatcaattt 361 catcaaagaa aataaggcag gctttttttg tcctggccat ttcaaaaagc tcacgaacca 421 ttcgagctcc ctcgccgacg tatttctgta cgagctcaga cccaataact cgaatgaagc 481 agcatcagtc cgattggaac tgccta //