LOCUS BG064511 659 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020F02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020F02 3', mRNA sequence. ACCESSION BG064511 VERSION BG064511.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 659) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064511.1. Other_ESTs: H3020F02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3020 row: F column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..659 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020F02-3" /db_xref="taxon:10090" /clone="H3020F02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 178 c 186 g 120 t ORIGIN 1 gtctcaactc ggcaaatttt atttagaaaa aatggtgggg tttgaaaaaa gtgacaccac 61 tcattacgta cggacttaga agcatccacc cttctgtagc accttaggct cttctctcag 121 tttaacctcg cacaacttgt agagaaaagg tcaagtcccc tgccgaagca gagtggatgg 181 cggaggtgct tgaaagcggc cagagaagag ccccaggcga tcgacgttgc ggtggaggac 241 actgtgcagc acagacagat gtggtcctcc ttcagctcca ccctccctgg caaagtcccg 301 gatgaagcgg ccacgttcag actggaagat gaacttctgg ggcaagggcc catgctcccg 361 aagaaaaccc cagacactaa gcagcaacag acgcacttca aaaggtgcca gttggctaaa 421 tacctcgtgc atattaccca gggctggtgg caagaggctt ccctcagcca tgacagccac 481 caggatacag gatgcctcca gatgccaggg ggagtgggtt gtatcagggt gtcgagaggc 541 ttcccaatgg ctcaagaggg cagccagcag cccccgcagc agcacggagc agtagcacag 601 ggctggggga gcagctgcta ccagcttcaa aagctcaaac agcagaggtt gttcccgga //