LOCUS BG064374 668 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018G11 3', mRNA sequence. ACCESSION BG064374 VERSION BG064374.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 668) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064374.1. Other_ESTs: H3018G11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: G column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..668 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018G11-3" /db_xref="taxon:10090" /clone="H3018G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 138 c 130 g 218 t ORIGIN 1 aaacttaaaa gggatttatt tgtgatttcc tatatatatt tagcttgtaa atacaagact 61 gtaaatgtat taagagacaa tttctgttaa agttttcatt gtgtttcact tcaagtactg 121 cacaagttaa aatctgataa aggatttaca ttcgggtatc tgaaactcct catctcagac 181 ttctgtttca tgtggtgata acttcatctc catagacacc accagcagga aagtctctcc 241 tatggcttct tgcactttcg ttaattagtg tgcatacagt tttcattttc taggtcattg 301 tcattatcat tgctgtcttc gtcactttcc agggggatac ctgtggcagc tgaagcacct 361 ttagtttctc ggtcaagagg gaagaagcca gtcccactga aagtgtcctg tctctggtag 421 aagagcacgt acgctgcctt ggacacgatt tggtcttcag atgcagtgga gacgctactg 481 tcatcgaaat agtaccactt cccatcatct ttgttttttg caaaagcagt ataatgtcct 541 cctcccatcc ctccatagtg gttggaaaca gcaatcaaat tatagcggca aggacctgca 601 tttgggttaa ttaggaattc tgacatatcc aagtcactga tgggaaaatc gactaatgta 661 tctagctt //