LOCUS BG064371 562 bp mRNA linear EST 12-MAY-2010
DEFINITION H3018G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3018G08 3', mRNA sequence.
ACCESSION BG064371
VERSION BG064371.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 562)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064371.1.
Other_ESTs: H3018G08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3018 row: G column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..562
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3018G08-3"
/db_xref="taxon:10090"
/clone="H3018G08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 110 a 166 c 163 g 123 t
ORIGIN
1 cgataagaaa acatctttat tttaaatact ttggaaagct taatcatcag tgcctggggg
61 aggctatggt cccagacccc ctggcctccc catccccaag gctctgggga tcacatgagc
121 tgctgaactc gggggggaga caggctggaa agacctggac ttctttctgg tcagtggaac
181 cagaggtctc ctgcctggag ggctgagcag acagctggca tttgaggtag ggtggcccca
241 gagtgtgagg gtacagggtc ctgtggagac tggaaagcag gcagctgctc ttttctggtc
301 catggtaacg aggtcctcag gcctgcgccc caaagcccga agggttctgc ttctgccagc
361 gccacagatc ttcacacatc ctgtccagcc ccagggctgc tgtccagccc agctcctcat
421 gggccaggct ggggttggca taacaggccg ccacatcacc ttcccgccgt gccaccacct
481 tgtacgggat cttcttccct gaggctttct ccatggcttg gaccatctgc aggacagagt
541 agcctgtgcc cgtgcccagg tt
//